兔抗小鼠IgA抗體Mouse IgA相關(guān)標記     Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647   AP   APC   Biotin   Cy3   Cy5  
Cy5.5   Cy7   FITC   Gold   HRP   PE   PE-Cy3   PE-CY5   PE-CY5.5   PE-CY7   RBITC  
英文名稱    Mouse IgA
中文名稱    兔抗小鼠IgA抗體
別    名    A2m marker; FLJ14473; FLJ35065; FLJ35500; FLJ36402; FLJ39698; FLJ40001; FLJ41548; FLJ41552; FLJ41789; FLJ43248; FLJ43594; FLJ44293; FLJ46028; FLJ46621; FLJ46724; FLJ46811; FLJ46824; Ig alpha 1 chain C region; Ig alpha 2 chain C region; IgA1; Igh2; IGHA 1; IGHA 2; IGHA1; IGHA2; Immunoglobulin alpha 1; Immunoglobulin Am1; Immunoglobulin Am2; Immunoglobulin heavy chain 2 (serum IgA); Immunoglobulin heavy constant alpha 1; Immunoglobulin heavy constant alpha 2; MGC102857.
研究領(lǐng)域    細胞生物  免疫學(xué)  
抗體來源    Rabbit
兔抗小鼠IgA抗體Mouse IgA克隆類型    Polyclonal
交叉反應(yīng)    Mouse, 
產(chǎn)品應(yīng)用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）  not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量    55kDa
細胞定位    分泌型蛋白 
性    狀    Lyophilized or Liquid
濃    度    1mg/1ml
免 疫 原    Mouse IgA protein purified
亞    型    IgG
純化方法    affinity purified by Protein A from plasma
儲 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed    PubMed
產(chǎn)品介紹    background:
Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.

Subcellular Location:
Secreted

Gene ID:
3494

Database links:
Entrez Gene: 3494 Human
Entrez Gene: 5284 Human
Omim: 146900 Human
Omim: 147000 Human
Omim: 173880 Human
SwissProt: P01876 Human
SwissProt: P01877 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-Mouse IgA Polyclonal Antibody, Unconjugated(bs-0774R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining