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RBP4 Human, His/ 重組人*結(jié)合蛋白-4, His

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RBP4 Human, His/ 重組人*結(jié)合蛋白-4, His 產(chǎn)品編號：CYT-555
產(chǎn)品規(guī)格：2μg/10μg/1mg

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RBP4 Human, His/ 重組人*結(jié)合蛋白-4, His

產(chǎn)品編號：CYT-555
產(chǎn)品規(guī)格：2μg/10μg/1mg

Synonyms	Retinol Binding Protein 4 plasma, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP.
Introduction	Retinol binding protein 4（RBP4） belongs to the lipocalin family and is the specific carrier for retinol （vitamin A alcohol） in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the rbp-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
Description	RBP-4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids and having a molecular mass of 23 kDa. RBP4 is fused to N-terminus His tag which containes additional 21 amino acids. RBP4 sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 amino acids 16–199. The Retinol Binding Protein-4 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	White lyophilized （freeze-dried） powder.
Formulation	RBP-4 His tag was filtered （0.4 ?m） and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5.
Solubility	It is recommended to reconstitute the lyophilized Retinol binding protein-4 in sterile deionized water to a working volume of 0.5mg/mL and let the lyophilized pellet dissolve compley. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability	Lyophilized Retinol binding protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Retinol binding protein-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Biological Activity	Determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5M retinol acids/per M RBP4.
Amino acid sequence	MSWWHHHHHH NWNIPTTQDT TERDCRVSSF RVKENFDKAR FSGTWYAMAK KDPEGLFLQD NIVAEFSVDE TGQMSATAKG RVRLLNNWDV CADMVGTFTD TEDPAKFKMK YWGVASFLQK GNDDHWIVDT DYDTYAVQYS CRLLNLDGTC ADSYSFVFSR DPNGLPPEAQ KIVRQRQEEL CLARQYRLIV HNGYCDGRSERNLL.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.