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IGFBP 3 Human/ 人胰島素樣生長因子結(jié)合蛋白-3

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IGFBP 3 Human/ 人胰島素樣生長因子結(jié)合蛋白-3 產(chǎn)品編號：CYT-300
產(chǎn)品規(guī)格：5μg/25μg/1mg

詳細介紹

 

IGFBP 3 Human/ 人胰島素樣生長因子結(jié)合蛋白-3

產(chǎn)品編號：CYT-300
產(chǎn)品規(guī)格：5μg/25μg/1mg
 

Synonyms Growth-hormone-dependant binding protein, IBP3, BP-53, IGFBP-3.
Introduction IGFBP3 is a member of the insulin-like growth factor binding protein （IGFBP） family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit （IGFALS） and either insulin-like growth factor （IGF） I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Description IGFBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 2x264 amino acids and having a molecular mass of 28806 Dalton.
IGFBP-3 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation The protein was lyophilized with 20mM Tris HCL.
Solubility It is recommended to reconstitute the lyophilized Insulin-like Growth Factor Binding Protein-3 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized IBP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution IGF-BP 3 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity Greater than 98.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Ala-Ser-Ser-Gly.
Biological Activity The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.2 μg/ml in the presence of 15 ng/ml of Human IGF-II.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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