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【文獻(xiàn)標(biāo)題】Lentivirus expressing soluble ST2 alleviates bleomycin-induced pulmonary fibrosis in mice
【作者】Qiaoyan Gao, Yan Li,Xiuhe Pan,et.al
【作者單位】寧波大學(xué)(Ningbo University)
【文獻(xiàn)中引用產(chǎn)品】
小鼠羥脯氨酸(Hyp)ELISA試劑盒
【關(guān)鍵詞】Interleukin-33,ST2,Lentivirus vector,Inflammation,Collagen,Pulmonary fibrosis
【DOI】dx.doi.org/10.1016/j.intimp.2015.11.015
【影響因子(IF)】5.1
【出版期刊】《International Immunopharmacology》
【產(chǎn)品原文引用】
Hydroxyproline assay
The hydroxyproline level was used to examine the content of collagen in the lung homogenates. The lungs were harvested 28 days after treatment and homogenized in PBS (pH 7.2–7.4). The supernatant was collected and aliquot after centrifuged at 3000 g for 15 min under 4 °C.The concentration of hydroxyproline was detected with Mouse Hyp kit (Jinma Biological Technology Co. Ltd., Shanghai, China) as the specification.
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