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pRNAT-H1.1/Shuttle

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所在地區(qū)：上海上海市

聯(lián)系人：李小姐

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產(chǎn)品簡介

pRNAT-H1.1/Shuttle is an adenoviral siRNA shuttle vector. It is compatible with the Clontech Adeno-XTM Expression System 1. It carries a GFP marker (coral GFP, cGFP)

詳細介紹

Description: pRNAT-H1.1/Shuttle is an adenoviral siRNA shuttle vector. It is compatible with the Clontech Adeno-X^TM Expression System 1. It carries a GFP marker （coral GFP, cGFP） under CMV promoter control. This marker can be used to track the transfection efficiency. It uses an H1 promoter for siRNA expression.

Download Protocol:tm0165

Forward Sequencing Primer:
DA0013:pRNA-H1 Forward （TAATACGACTCACTATAGGG）

Reverse Sequencing Primer:
DA0012:pRNA Reverse （TAGAAGGCACAGTCGAGG）

Detailed Map:

Polylinker:6164-6187

H1 Promoter:6064-6163

CMV Promoter:37-624

cGFP:641-1357

SV40:2085-2430

Neomycin:2471-3265

pUC ori:3979-4619

Ampicillin:4767-5627

Publications used this GenScript Product:

Jiliang Zhou and B. Paul Herring. Mechanisms responsible for the promoter specific effects of myocardin. J. Biol. Chem. Jan 2005; 280 （11） :10861-10869

El-Mounayri OA, Triplett JW, Yates CW, Herring BP. Regulation of smooth muscle-specific gene expression by homeodomain proteins, Hoxa-10 and Hoxb-8. J. Biol. Chem. Jul 2005; 280 （27） :25854-25863

Feng Yin, April M Hoggatt, Jiliang Zhou, and B. Paul Herring. The 130kDa Smooth Muscle Myosin Light Chain Kinase is Transcribed from a CArG-Dependent, Internal Promoter Within the Mouse MYLK Gene. Am. J. Physiol Cell Physiol. Jun 2006; 290 （6） :C1599-1609

Nakerakanti SS, Kapanadze B, Yamasaki M, Markiewicz M, Trojanowska M. FLI1 and Ets1 have distinct roles in the CTGF/CCN2 gene regulation and induction of the profibrotic gene program. J. Biol. Chem. Sep 2006; 281 （35） :25259-25269

Pannu J, Nakerakanti S, Smith E, Dijke PT, Trojanowska M. TGF-beta receptor type Idependent fibrogenic gene program is mediated via activation of smad1 and ERK1/2 pathways. J. Biol. Chem. Apr 2007; 282 （14） :10405-10413

Yoshihide Asano, Joanna Czuwara-Ladykowska, Maria Trojanowska. TGF-β regulates DNA binding activity of transcription factor Fli1 by PCAF-dependent acetylation. J. Biol. Chem. Nov 2007; 282 （48） :34672 - 34683

Liling Yang, Shuxing Wang, Backil Sung, Grewo Lim, and Jianren Mao. Morphine induces ubiquitin-proteasome activity and glutamate transporter degradation. J. Biol. Chem. Jun 2008;