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技術(shù)文章

CD4 抗體說明書antibody

閱讀:321發(fā)布時(shí)間:2012-9-10

 Anti-CD4 抗體說明書
Catalog Number :  RS-0766R  
Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent )

Background:  The CD4 antigen is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. This integral membrane glycoprotein of approximay 58 kDa contains four external domains (D1 to D4) that show homology to members of the immunoglobulin (Ig) superfamily. It is responsible for the recognition of the MHC class II antigen. The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells.

pecificity :   
– anti- CD4 is a goat monoclonal antibody  unconjugated               
– specific for CD4 of rabbit              
– epitope mapping in the middle of  CD4 of rabbit origin
– Protein G affinity chromatography purification, purity :>95%
– Isotype:  IgG 
– se for western blotting, elisa, immunoprecipitation and    Immunohistochemistry
– mol wt: Recognizes 29 kDa
 Anti-CD4 抗體說明書
Application :   
–  Western blotting 1:100-500               
–  Immunohistochemistry 1:100-500               
–  ELISA 1:500-1000                 
–  Optimal working dilutions must be determined by the end user. 
Storage:        Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃.
Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

 Anti-CD4 抗體說明書
 


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