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英文名稱 Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon/FITC
中文名稱 FITC標(biāo)記的14-3-3蛋白/14-3-3 α/β/γ/δ/ε亞型抗體
別 名 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; KCIP 1; Protein 1054; Protein kinase C inhibitor protein 1; 14 3 3 protein delta; 14 3 3 protein gamma; YWHAB; YWHAZ; 14 3 3 protein Epsilon; 1433Z_HUMAN. 
說 明 書 100ug  
研究領(lǐng)域 細(xì)胞凋亡  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Sheep, Fruit Fly, yeast
產(chǎn)品應(yīng)用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 27kDa
性 狀 Lyophilized or Liquid
濃 度 2mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human 14-3-3 (201-245aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
FITC標(biāo)記的14-3-3蛋白/14-3-3 α/β/γ/δ/ε亞型抗體保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
FITC標(biāo)記的14-3-3蛋白/14-3-3 α/β/γ/δ/ε亞型抗體產(chǎn)品介紹 background:
14-3-3 are activates tyrosine and tryptophan hydroxylases in the presence of Ca (2+)/calmodulin-dependent protein kinase II, and strongly activates protein kinase C. Is probably a multifunctional regulator of the cell signaling processes mediated by both kinases. Activates the ADP-ribosyltransferase (exoS) activity of bacterial origin. 14-3-3 proteins are localized in neurons, and are axonally transported to the nerve terminals. They may be also present, at lower levels, in various other eukaryotic tissues. It belongs to the 14-3-3 family.
This antibody is reactive with 14-3-3 Alpha, Beta, Gamma, Delta, Epsilon.

Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subunit:
Interacts with CDK16 and BSPRY. Interacts with WEE1 (C-terminal). Interacts with SA1. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with BCL2L11. Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. Interacts with GAB2 and TLK2.

Subcellular Location:
Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.

Post-translational modifications:
The delta, brain-specific form differs from the zeta form in being phosphorylated. Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.

Similarity:
Belongs to the 14-3-3 family.

Database links:

Entrez Gene: 7529 Human

Entrez Gene: 286863 Cow

Entrez Gene: 54401 Mouse

Entrez Gene: 56011 Rat

Omim: 601289 Human

SwissProt: P68250 Cow

SwissProt: P31946 Human

SwissProt: Q9CQV8 Mouse

SwissProt: P35213 Rat

Unigene: 643544 Human

Unigene: 34319 Mouse

Unigene: 485025 Mouse

Unigene: 8653 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

信號傳導(dǎo)（Signaling Intermediates）
14-3-3蛋白是一個涉及調(diào)節(jié)細(xì)胞凋亡、促細(xì)胞分裂信號傳導(dǎo)和細(xì)胞周期關(guān)卡的蛋白質(zhì)家族。它被認(rèn)為是通過與*殘基磷酸化的蛋白質(zhì)的結(jié)合介導(dǎo)的信號傳導(dǎo)中的關(guān)鍵調(diào)節(jié)物。通過與Bad(相關(guān)死亡因子)的結(jié)合， 14-3-3 蛋白由于將Bad隔離于胞液而防止了細(xì)胞凋亡。
蛋白是14-3-3家族成員。它廣泛分布于哺乳動物、兩棲類、昆蟲、植物和酵母菌的真核生物高度保守性多功能蛋白質(zhì)。
目前已知至少有16個成員。此抗體識別分子量為30-31kDa的14-3-3蛋白αβγδε亞型。