中文名稱:人B細(xì)胞活化因子受體(BAFF-R)ELISA試劑盒
英文名稱:The human B- cell activating factor receptor (BAFF-R) ELISA Kit
規(guī) 格:96T/Kit (8*12 strips) 48T/Kit (8*6 strips)
保存和有效期:2-8℃/6個(gè)月
運(yùn)輸條件:低溫運(yùn)輸,用干冰或者冰袋低溫運(yùn)輸。
可測(cè)種屬:人、大小鼠、豚鼠、兔子、豬、犬、猴、馬、、、、、、
可測(cè)標(biāo)本:血清、血漿、細(xì)胞上清液、尿液、體液、灌洗液、、、、、、、
定義:一種酶聯(lián)免疫技術(shù),用于檢測(cè)被于固相板孔中的待測(cè)抗原(或抗體)。即用酶標(biāo)記抗體,并將已知抗原或抗體吸附在固相載體表面,使抗原抗體反應(yīng)在固相載體表面進(jìn)行,用洗滌法將液相中的游離成分洗除,zui后通過酶作用于底物后顯色來判斷結(jié)果。
人B細(xì)胞活化因子受體(BAFF-R)ELISA試劑盒專一性強(qiáng)、靈敏度高、樣品易保存。
應(yīng)用學(xué)科:免疫學(xué)(一級(jí)學(xué)科);應(yīng)用免疫(二級(jí)學(xué)科);免疫學(xué)檢測(cè)和診斷(二級(jí)學(xué)科)
人B細(xì)胞活化因子受體(BAFF-R)ELISA試劑盒必須遵守以下3個(gè)核心原理:
1)抗原或抗體能吸附于固相載體表面,并保持其免疫學(xué)活性;
2)抗原或抗體可通過共價(jià)鍵與酶連接形成酶結(jié)合物,而此種酶結(jié)合物仍能保持其免疫學(xué)和酶學(xué)活性;
3)酶結(jié)合物與相應(yīng)抗原或抗體結(jié)合后,可根據(jù)加入底物的顏色反應(yīng)來判定是否有免疫反應(yīng)的存在,而且顏色反應(yīng)的深淺是與標(biāo)本中相應(yīng)抗原或抗體的量成正比例的,可根據(jù)已知濃度的抗原或抗體的顏色反應(yīng)的深淺繪制標(biāo)準(zhǔn)曲線并依此計(jì)算出未知樣品中抗體或抗原的濃度。在ELISA中酶起到關(guān)鍵的作用,常用于ELISA法的酶有辣根過氧化物酶,堿性磷酸酯酶等,其中以辣根過氧化物酶為多,人B細(xì)胞活化因子受體(BAFF-R)ELISA試劑盒酶結(jié)合物是酶與抗體或抗原,半抗原在交聯(lián)劑作用下聯(lián)結(jié)的產(chǎn)物,它不僅具有抗體抗原特異的免疫反應(yīng),還能催化酶促反應(yīng),顯示出生物放大作用。
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