?產(chǎn)品名稱:人谷胱甘肽還原酶(GR)ELISA檢測(cè)試劑盒
?英文名稱:human Glutathione Reductase (GR) ELISA Kit
?產(chǎn)品規(guī)格:96T(人份)/48T(人份)(單孔檢測(cè)分別可以檢測(cè)90份樣本,42分樣本,一般都需要留6個(gè)孔做標(biāo)準(zhǔn)曲線)。
?檢測(cè)方法:酶聯(lián)免疫分析ELISA方法
人谷胱甘肽還原酶(GR)ELISA檢測(cè)試劑盒試驗(yàn)以靈敏度較高、特異性較好的特點(diǎn)在臨床上得到了廣泛的應(yīng)用,但操作中的各個(gè)環(huán)節(jié)對(duì)試驗(yàn)的檢測(cè)效果影響較大,如不注意,有可能導(dǎo)致顯色不全、花板等結(jié)果。我將操作中各個(gè)環(huán)節(jié)常出現(xiàn)問題的原因及解決辦法總結(jié)于下,以期給同行帶來一些啟發(fā),提高試驗(yàn)質(zhì)量。
下面分析Elisa試驗(yàn)操作中可能影響結(jié)果的原因,并給出相應(yīng)的解決辦法
1 、選擇試劑
選擇質(zhì)量優(yōu)良的檢測(cè)試劑,嚴(yán)格按照試劑說明書進(jìn)行操作,操作前將試劑在室溫下平衡30-60分鐘。
2 、加樣
可能原因: 1)血清或血漿標(biāo)本分離不好即進(jìn)行加樣; 2)手工操作中,加樣板過多造成加樣后放入孵箱前等待時(shí)間過長(特別是室內(nèi)溫度較高時(shí)); 3)加完標(biāo)本再加酶試劑時(shí)酶濺出孔外。
解決辦法: 1) 標(biāo)本為血清:將血液先自然存放1-2小時(shí)后,再用3000rmp離心15分鐘;標(biāo)本為血漿:必須使用含抗凝劑的血液標(biāo)本收集管,采血后必須立即顛倒采血管混合5-10次,放置一段時(shí)間后,3000rpm離心15分鐘;若在幾天內(nèi)檢測(cè),可放在2-8℃冰箱中,若要貯存,則置于-20℃的低溫冰箱內(nèi)。 2) 加樣后及時(shí)放入孵箱。 3) 加酶試劑后用吸水紙?jiān)诿笜?biāo)板表面輕拭吸干。 4) 如果采用AT或其他全自動(dòng)加樣,選擇FAME或其他后處理儀器加酶試劑。 5) 標(biāo)本較多時(shí),請(qǐng)分批操作。
3 、孵育
可能原因: 1) 孵育時(shí)未貼封片或加蓋,使標(biāo)本或稀釋液蒸發(fā),吸附于孔壁,難于清洗*; 2) 孵育時(shí)間人為延長,導(dǎo)致非特異性結(jié)合緊附于反應(yīng)孔周圍,難以清洗*。
解決辦法: 1) 貼封片或加蓋; 2) 按說明步驟嚴(yán)格控制操作時(shí)間。
4 、洗板
可能原因: 1) 采用手工洗板,孔與孔之間液體交叉。 2) 采用半自動(dòng)洗板機(jī)洗板時(shí),洗液量不足,導(dǎo)致洗板不*;洗板針堵塞,抽吸不*;洗板不暢,導(dǎo)致洗板效果差。 3) 反應(yīng)板過多造成洗板等待時(shí)間長。
解決辦法: 1) 保證洗液注滿各孔,洗板針暢通,洗完板后在吸水紙(選擇干凈、無或少塵的吸水材料)上輕輕拍干; 2) 合理安排,或多用幾臺(tái)洗板機(jī)。
5 、顯色
可能原因: 1) 顯色劑配制后放置時(shí)間過長或使用過期顯色劑; 2) 加顯色劑時(shí)濺出孔外造成液體回流。
解決辦法: 1) 顯色劑盡量在臨用前配制,堅(jiān)持不用過期顯色劑,肉眼可見淺藍(lán)色的TMB顯色劑不用; 2) 加樣時(shí)保持顯色劑不外流; 3) A、B液應(yīng)避免接觸金屬器械。
6 、終止
可能原因如加終止液時(shí)產(chǎn)生較多氣泡,導(dǎo)致假陽性增加。所以在加終止液時(shí)應(yīng)避免產(chǎn)生氣泡。
7 、讀板
如讀板時(shí)板底不清潔等。應(yīng)保證酶標(biāo)板清潔。
所以在整個(gè)操作過程中保證酶標(biāo)板不接觸次氯酸; 盡可能實(shí)現(xiàn)ELISA檢測(cè)標(biāo)準(zhǔn)自動(dòng)化,有效提高檢測(cè)質(zhì)量。
在實(shí)際操作中,除了選擇優(yōu)良試劑外,必須嚴(yán)格按照操作步驟進(jìn)行操作,同時(shí)作好室內(nèi)質(zhì)控、室間質(zhì)評(píng),以嚴(yán)謹(jǐn)?shù)墓ぷ髯黠L(fēng)檢測(cè)每一份標(biāo)本,才能保證檢測(cè)質(zhì)量。深圳子科生物已經(jīng)用于9年的技術(shù)研發(fā)經(jīng)驗(yàn),能極大的保障每個(gè)消費(fèi)者的售后技術(shù)服務(wù),讓您買的放心,用的放心!
人谷胱甘肽還原酶(GR)ELISA檢測(cè)試劑盒9017850 USB Stick
9017846 Shaker Adapter, 2.0 ml, screw cap tubes
9017815 Board, gripper, QIAC
9017734 Plasticware kit, IQ/OQ, EZ1
9015212 Z-Module 0.9mm Probe, BR8 RD
9015179 Cooling Block 48-tube, 2.0 ml, M48
9015155 Transportation kit, BR8000
9015083 Light Barrier, X-Dri., SamTrack, BR8 RD
9238475 2ml O-ring adapter tips, RapidPlate
9238239 Gasket, auto vac, SBS top BR8000/BR3000
9238224 Alignment Microspheres (10ml)
9240666 Nut, Flangeless,1/4-28, Cream, AP
9240661 Nozzle Kit, Dispense Head, AP
9240652 Vacuum Pump, Gast, 120V, AP96
9240622 Circuit Breaker, 8A, AP196/98
9240608 Fiber Optic Sensor, Main unit, AP98
9240575 Cable/Sensor,Purge Bottle, X26, AP96/196
9240569 ZLV Drawer Slide,Full Ext,Centrigue,AP96
9240567 Driven Gear, Centrifuge Locator, AP96
9240532 Cable, Shaker Encoder, AP98
9240509 Hard Drive, Programmed, Win XP, AP196/98
9239962 Upgrade App., BR MDx DSP CTM 48 PCR
9239562 Assy, Fan Mounting, 60 CFM (Coherent)
9019981 Vacuum test plate
9019484 Gasket, Carriage upper, CX1
9241210 RCS, Premium Cover Platform-only,2PM,1yr "This service agreement supplements the service coverage for the manual digene Hybrid Capture system) after the upgrade to a Rapid Capture System.
The Premium Cover RCS platform-only, 2 PM provides full service coverage for the Rapid Capture System. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes two annual preventive maintenance visits, with costs for labor and parts covered. With a Premium Agreement, there is a priority response time of 24 hours (1 working day)."
9241207 QIAsymphony SP, Full Agreement The Full Agreement QIAsymphony provides full service coverage for the QIAsymphony System. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9241189 User Manual, QIAxcel 01/08 (WW)
9237330 Upgrade, BR & 4 ext. devices w. sched. Upgrade BioRobot with four approved integration devices using scheduling software
9237169 Bottle Waste
9233214 Tubing, waste, PVC, 8/12, (per m)
9237125 Upgrade App., DNeasy 96 "Take advantage of our instrumentation and molecular biology expertise to install
DNeasy 96 on your BioRobot System. The onsite protocol development visit includes
all travel and labor for the visit to install the standard QIAGEN DNeasy protocol, Plus a
DNeasy 96 Tissue Kit (4-plate) SAP# 69581"
9237107 Protocol Dev./Opt., on-site (3-days) The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel and labor charges for a site visit up to 3 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, and accessories not included. Custom protocols are the sole responsibility of the customer and neither warrantied nor guaranteed.
9232993 PCB, YZ-Connection, BR8
9232971 Syringe pump Board
9236410 Stacker, deepwell, TWI
9236362 Fuse 2A, 250VAC, Fast Acting
9236356 Sample Needle, long
9236352 Tubing, Valve to Pump
9236335 Sensor Distribution Board
9236157 User manual, RapidPlate
9236150 Locator Plate, 96 well&Reservoir,BR RP
9236148 Locator Plate, 384 well, BR Rapid Plate
9230679 Rack for reagents, Trough IBG
9021123 Valve Manifold, Upper, DCU
9021102 Skin Panel, Lower Left Side, DCU
9021049 Set, transparent panel, Qsym
9021026 Set, cables, transfer module, Qsym
9021012 Controller, Z Axis, VR, DCU
9020993 Pipettor Assembly, DCU
9020907 Cover, HEPA filter sys, SMC, QGX
9020905 UV light assembly, SMC, QGX
9020780 Notebook 1 XP
9020701 Clamp, table, single sided, C/S, C42
9020688 Cover - Bottom, PyroQ24
9020682 Pinch valve, PV02, QXT
9020640 Baseboard, front, AS, Cabinet Qsym
9020639 Baseboard, front, SP, Cabinet Qsym
9020365 Optical sensor, AS, Qsym
9020336 Pipetting head, 1 ch. (EXCHANGE), Cx2
9020315 PM kit, C12 & QIAgility (UV/HEPA), CG1